Tsapenko P. Influence of substance P on functional state of hepatocytes under in vivo and in vitro conditions

Object: peptide regulation of the functional state of hepatocytes. Objective: To explore the impact of the way of substance P on the functional state of hepatocytes and find out about their relatives tachykinin peptides (vasopressin and bombesin) in the implementation of this influence. Methods: physiological (acute experiments on rats, microelectrode techniques), biochemical (TLC) and the methods of statistical analysis. Results: It was established that muscarinic acetylcholine receptors are not involved in the implementation of the stimulating effect of substance P on bill secretion of liver. In addition, research conducted electrical activity of hepatocytes in vitro using microelectrode technique for the prepared parts of the liver under the influence of substance P, bombesin and vasopressin. Showing the effect of the studied peptides in the membrane potential of hepatocytes. Shown that the substance P in a concentration of 1 mkM has no effect on the membrane potential of hepatocytes. Perfusion of the liver with a solution of the drug vasopressin causes hyperpolarization of membranes of hepatocytes. Bombesin, unlike vasopressin, causes depolarization of the cell membrane of rat liver parenchyma. Bombesin and vasopressin exercise their influence through the activation of protein kinase C, however, there are other ways of action of these peptides. Prostaglandins are not involved in the implementation of bombesin effects on membrane potential of hepatocytes, since the action of bombesin on the background of aspirin did not differ from those without the blocker. Application areas: physiology, pharmacology.