Makhno Y. Identification of oil flax collection and breeding material using electrophoresis of seed storage proteins

The object of study - the method of electrophoretic distribution of seed storage proteins and identification of oil flax breeding material based on protein polymorphism. Purpose - to develop a method of electrophoretic analysis of components of oil flax storage proteins source material for use in the breeding process to create new and original varieties. Methods - field, mathematical, statistical, laboratory. Results - the method of electrophoretic distribution of oil flax storage proteins in acidic polyacrylamide gels was developed. We established specific polymorphism of electrophoretic specters in oil flax collection and breeding material in position, intensity, presence or absence of protein components. High degree of heterogeneity in the hybrids of the first, second and third generations in intraspecific hybridization was detected. We created a catalog of protein components and provided a detailed description of 22 oil flax genotypes as protein formulas and positions of protein components on graphics. Novelty - for the first time an effective method of electrophoretic distribution of seed storage proteins in acidic polyacrylamide gels was developed for oil flax and on its basis a method of identifying varieties of oil flax with its protein components was developed as well. We recommend to use electronic catalog of oil flax protein formulas as a basis for screening breeding material, defining originality and typicality of samples, controlling constancy and purity of commercial varieties at different stages of seed development. Sector - agriculture.