Ivanenko M. Development of I. I. Mechnikov cellular immunity concept in research of resistance mechanisms and inflammation progress at experimental samples

The object of study: The changed immune physiological responses at induced inflammatory processes in animal experiments and in the patients with intestinal ischemia. Purpose of the research: Evaluation of the peculiarities of interaction between primary cellular and secondary humoral immune physiological resistance factors at the induced inflammatory process at experimental models according to I. І. Mechnikov concept about the mechanisms of action of cellular immunity induction and inhibition factors. Methods: Light microscopy, spectrophotometry, immune turbidimetry, statistical analysis.Theoretical and practical results, novelty: For the first time the comparative study has been done for the parameters of primary and secondary immune resistance links; these results could be used at integral approaches to the prevention of complications.It has been revealed that at experimental peritonitis in the older group of animals the phagocytic index decreases compared to the controls that agrees with I. I. Mechnikov concept about the changed phagocytic activity in ontogenesis. It has been demonstrated that enzyme activity of NADH-oxidase which in presence of oxygen active forms (OAF) provides endocytosis, is more intensive in the animals of different age with experimental peritonitis model compared to the control rats that agrees with I. I. Mechnikov theory principles which ground the role of inflammation factors. Examination of immunoglobulin A concentration in blood serum of animals of different age at the inflammation model demonstrated its reduction that is explained by I. I. Mechnikov concept about the role of serum factors which induce phagocytic neutrophils activity. It has been revealed that at intestinal ischemia against the background of mesenterial thrombosis the phagocytic index decreases in the patients of the older age group compared to the controls that agrees with I. I. Mechnikov "orthobiosis" theory about the failure of intestinal probiota and detoxication of the organism. The reliable increase of immunoglobulin A concentration in blood serum of the junior age group and circulating immune complexes (CICs) increase in both age groups of the patients with intestinal ischemia. Field of use for the results of the work: The proposed approaches to diagnostic examination may be used while establishing metabolic immune reserves in organisms of different age as well as for the choice of the methods of target immune correction considering the age characteristics.