Nakonechnaya S. Evaluation of the effect of chronic influence of phenol derivatives on alteration in physiological states in experimental animals.

Research object is the physiological state of animal organizm. Purpose is determination of functional activity of some regulator systems of organism (simpato-adrenal, hemopoesis, pro antioxidant, system of microminerals under chronic influence of derivatives of phenol based on conception of allostasis. Research methods are a modelling of the physiological states, biochemical, chromatography, spectrophotometry, atomic-absorbings and statistical.The thesis is devoted to the peculiaritiesof physiological states in experimental animals as a response to the chronic effects of ionic and nonionic neonols possessing surface-active properties, which are widely used in everyday life. It is shown that neonols AP 9-12 and APS 6-9 CM in doses of 1/10 and 1/100 DL50 negatively affect the organism on the primary stage of the experiment. They cause decrease in weight gain, various manifestations in the redistribution of macronutrients and micronutrients in organs and tissues. These phenomena lead to a shift in activity of metalloenzymes in serum and membrane-bound enzymes. Neonols trigger mechanisms of lipid peroxidation, leading to activation of antioxidant enzymes in the cells, structural and metabolic changes of cell membranes and changes in the morphological state of the blood. APS 6-9 CM (ionic surfactants) has a more pronounced effect. Reducing percentage of deviations from the normal functional state of the animals that had been caused by the impact of exogenous load was revealed during the experiment. Activation of cellular resources for restoring of structures was detected at the end of the experiment. Varied sensitivity of organs to the action of neonols is identified. It is shown that the prolonged exogenous influence of both neonols causes stabilization of the organism state, which can be estimated as allostatic type of response. A practical value consists of that materials of researches can be used for making of informative documents and methodical recommendations, development of diagnostic protocols of estimation of action of xenobiotics, and also in an educational process.