Mozgovska H. Creating source initial material for breeding of eggplant for resistance to Fusarium wilt and heat resistance on the basis of biotechnological methods

Object: the isolated cells in culture of breeding of eggplant for resistance to Fusarium wilt and heat resistance. Purpose: theoretical and experimental study on the use of modified complex laboratory methods of cell selection, embryo and induced mutagenesis to create sustainable wilt disease and heat resistance of the linear material of eggplant. Methods: the methods of culture of organs and tissues; mycological; field studies; measuring and weighing; analytical; processing statistical data. Novelty: created four lines of eggplant with resistance to Fusarium wilt and heat resistance; a method of producing plant lines of the vegetable family of Solanaceae L. in a culture in vitro; developed a way to create original material of eggplant resistance to Fusarium wilt; developed a method to overcome the incompatibility of eggplant. Results: created four lines of eggplant S. m. 62, S. m. 63 with resistance to Fusarium wilt of 7 points; S. m. 79, L 21, with a degree of heat resistance of up to 90.1%; found that on MS medium supplemented with 0.1 and 0.1 GK3 0.1 NAA initiation of germination of immature embryos is 97.7%; effective dose of gamma irradiation of seeds of eggplant should not exceed 161.8 Gy; the evaluation of the heat resistance of eggplant samples is 76.3%. The degree of implementation: reference implementation in breeding programs of the Institute of vegetables and melons of NAAS, Donetsk OBI of NAAS experimental station, Crimea Institute of agriculture of NAAS, in the educational process of the Kharkov national agrarian university of V.V. Dokuchaev, State institution of higher education "Ukrainian state university of chemical technology." Scope: for breeders research institutions.