Bukreeva N. DNA markers of the quantitative traits loci in the study of maize heterosis

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0414U006049

Applicant for

Specialization

  • 03.00.15 - Генетика

26-12-2014

Specialized Academic Board

Д 41.363.01

Essay

Object: heterosis of maize performance. Aim: mapping QTL of maize performance and modeling MAS prediction of parental genotypes in maize populations for receiving high-yield hybrids and efficiency of the selection process. Methods: SSR-analysis, biometrics and genetic-statistical modeling, methods of evaluation grain yield in maize. Novelty: a scheme of molecular marker analysis, which can improve the efficiency of selection of genotypes in populations of recombinant inbred lines for receiving high-yield hybrids and allows to reduce the volume of variety testing, and identified DNA markers QTL «grain yield" in maize hybrids. Results: the model of DNA prediction of potentially valuable genotypes have been proposed based on the formation of classes in the populations of F4, F6 recombinant inbred lines by the genetic distance as to tester lines Odes’ky 308 MV, Odes’ky 221 MV and Odes’ky 329 and the blocks was separated with positive correlations between genetic distances of the recombinant inbred lines of different congeneric groups and «grain yield» of the obtained hybrids. The loci of the trait «grain yield» was marked and SSR-markers of QTL was detected which influence on the formation of the trait «yield of grain» of maize under different environments. Application stage: to create a simple modified high-yield hybrid Dialog 300. Application area: SIE of NAAN, research establishments and high school of MES and NAS of Ukraine.

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