Mosula M. Population-genetic analysis of yellow gentian (Gentiana lutea L.) from the Ukrainian Carpathians

The thesis is devoted to the investigation of Gentiana lutea L. status from the Ukrainian Carpathians by studying the level of genetic polymorphism and substantiation of approaches to conservation of this species. The features of genetic structure and level of diversity have been investigated for G. lutea populations from the two mountain chains of the Ukrainian Carpathians using five types of DNA-based markers (RAPD, ISSR, RGAP, CDDP, IRAP). Based on the analysis of the populations' genetic structure and comparison of these results with ecological characteristics (area, density, age, spatial and vitality structure, ability of renewal and self-maintenance) the status of species populations has been assessed. Thus, the necessity of their renewal has been established. The recommendations for the protection and conservation of G. lutea populations have been developed. The effective indicator of the primers informativeness has been selected with intention to evaluate the level of populations' genetic diversity. The system of PCR-based primers has been worked out in order to optimize the study of gene pools of G. lutea populations. Key words: Gentiana lutea L., genetic structure, genetic diversity, differentiation of populations, populations' status, DNA-markers, discriminating power (DL), the system of PCR-based primers.