Sapko S. Molecular diagnosis of tuberculosis of livestock and poultry (improvement of sample preparation and identification of epizootic important species of mycobacteria).

Thesis is devoted to assessing the impact of various chemical compounds and substances, with different concentrations and exposure on the functional integrity of the bacterial DNA for various types of research in the PCR. The effectiveness of different methods of extracting mycobacterial DNA, and a method of DNA extraction from animal tissue for genetic analysis using the polymerase chain reaction, was developed. Bioinformatics analysis of genes, insertion segments and the other portions target allowed developing specific molecular genetic techniques indication mycobacterial DNA and the species M. bovis and M. avium complex using the polymerase chain reaction. A test system for the detection of DNA of Mycobacterium avium complex "DNA-Test-MAC" (TU U 24.4-00497087-079), which is based on constructed primer pair AVF/AVR and amplification of the gene 16S pDNA length 193 bp. Developed and conducted laboratory trials of the test system to indicate Mycobacterium bovis based on polymerase chain reaction (TU U 21.2-00497087-154), with the primer pair bov 21/bov 22 that flank the site of the gene RvD1Rv2031c in length 500 bp.