Bogulska S. Creation of initial material of some agricultural crops resistant to herbicide with phosphinothricin as an active ingredient

Object: breeding technologies of making of initial material. Aim: establishment of initial material of winter and spring rape, sugar beet, corn and sunflower by in planta method. Methods: genetic analysis, crossbreed analysis, statistical analysis. Novelty: it was established that the optimal time of processing with agrobacterial suspension of the flowers of winter and spring rape, sugar beet, corn and sunflower is one minute. The efficiency of the method of plant transformation by the agrobacterial suspension has been proved, whereby at first pollen is applied to the stigmas of flowers and after germination of pollen tube they are treated with the bacterial suspension, thanks to which the level of seed formation rises. The method of sterile plants control in areas of hybridization was developed and the way of control the plants hybridity, where the resistance to herbicide with phosphinothricin as an active ingredient is used as a marker, was explained. Results: it was found that resistance to the herbicide is transmitted by hybridization and is inherited as a dominant trait. In sterile transformants Т1 after pollination the ratio between unstable plants that died and resistant plants is 1:1, but in plants of fertile genotypes Т1 after self-pollination – 3:1 respectively. It was found that under transformation of fertile plants with bisexual flowers Agrobacterium tumefaciens by in planta method the efficiency of transformants obtaining is lower than while using the process of pollination-fertilization by sterile plants. Application stage: the 4 patents of Ukraine were founded. Application area: SIE of NAS of Ukraine, NAAN of Ukraine.7452