Demidenko I. Isolation and cloning of porcine enteroviruses

The thesis presents the results of isolation and cloning of different porcine enteroviruses serotypes. Presented opportunity cultivation porcine enteroviruses in heterologous cell culture of Hep-2. proposed allocation clones porcine enteroviruses using the method of plaques. Through research carried differentiated characters in comparison of selected clones of isolates and reference strains of porcine enteroviruses. so studied the morphological characteristics of the selected clones by markers S, pt, pf, ps, pr; selected clones evaluated on reference strains/isolates of porcine enteroviruses by marker Ts (thermo resistant); set the degree of attenuation of selected clones using marker rct (thermal sensitivity); determined the immunespecificity of serum blood of rabbits obtained to reference strains, isolates and clones and set the degree of dominance selected clones of the original strains/isolates. Therefore, in the experiment conducted was found that one strain of porcine enteroviruses that may select by the method of breeding cloned variants, that are antigenically related with the original strain, but more resistant to various factors and have a higher antigenic activity.