Plotnikova L. The effect of partial oxygen pressure and hydrogen sulphide donor on the human somatic cells line 4BL proliferation.

This research focuses on proliferation of human somatic cells line 4BL under reduced partial oxygen pressure and/or the addition of hydrogen sulfide donor. It is shown that moderate intermittent hypoxia (Po2 38 and 76 mm Hg, 8 hours/day, 3 days) stimulate cell proliferation investigated line. The sharp decrease in oxygen partial pressure (23 mm Hg) in continuous mode (24 hours) inhibits the proliferation of human somatic cells line 4BL. Established that hydrogen sulfide donor NaHS in high concentrations (10-8 - 10-10 mol/l) has cytotoxic effect on cell culture and in low (10-12 - 10-15 mol/l) - inhibits their proliferation. The moderate hypoxia stimulate the growth of a culture of human somatic cells line 4BL, and the combined effect of hypoxia and H2S is not conducive to the physiological cell proliferation. The decrease in the partial oxygen pressure increases the inhibitory H2S effect on the proliferation. And hypoxia, and H2S reduce the alkaline fosfataty activity that may be due to inhibition of metabolic rate of cells. Reducing the partial oxygen pressure does not alter the morphology of human somatic cell line 4BL. The presence of H2S attached fibroblast-like cells of atypical forms. Found that both morphological and physiological characteristics of cell activity line 4BL, obtained from human peripheral blood, depend on the level of Po2 and the presence of hydrogen sulphide donor.