Goncharova K. The influence of exocrine pancreatic insufficiency and its correction on the neurons of CA1 hippocampal area

The thesis deals with the study of the effects of exocrine pancreatic insufficiency and its correction on the formation of hippocampal morpho-functional peculiarities in experimental animals. The research was carried out on the two animal species - domestic swine and Mongolian gerbil. The artificial exocrine pancreatic insufficiency was induced by pancreatic duct ligation. Newborn piglets and old gerbils were used for modeling of age-related exocrine pancreatic insufficiency. It was shown that artificial feeding under the physiological inactivation of pancreatic enzymes causes negative changes in the processes of neuronal and microglial migration in the hippocampus of newborn pigs. Also it was demonstrated that exocrine pancreatic insufficiency ameliorates behavioral reactions both in pigs and gerbils, leads to the decrease in the number of pyramidal neurons and causes destructive ultrastructural changes. All mentioned changes could be caused by maldigestion and malabsorption of nutrients. The data obtained allow to suggest that both short- and long-term replacement therapy with pancreatic-like enzymes of microbial origin corrects the deficits in behavior and structural changes in the CA1 hippocampal area both under the artificial and physiological exocrine pancreatic insufficiency.