Komisarenko A. The genetic transformation with dsRNA - suppressor of proline dehydrogenase gene for improving osmotoleration of sunflower (Helianthus annuus L.)

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0416U004324

Thesis Registration Form

0416U004324.pdf

Applicant for

Specialization

  • 03.00.15 - Генетика

13-10-2016

Specialized Academic Board

Д 26.212.01

Insninute of plant physiology and genetics

Essay

In the thesis are considered the problem of the development of appropriate methods for obtaining sunflower forms with high level of osmotolerance via Agrobacterium - mediated transformation using LBA4404 strain harboring binary vector pBi2E with dsRNA - suppressor of proline dehydrogenase gene. The original procedure of Agrobacterium - mediated transformation in vitro was worked out. The morphogenic ability activation factors were detected. The factors that promote the high frequency of DNA recombinant molecules integration were investigated too. First there was created the method of H. annuus Agrobacterium - mediated transformation in planta. The progeny (T1-T3) from transgenic lines were obtained.

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