Nikolaenko J. Epizootological monitoring of bird reovirus infection and the elaboration of its specific prevention in chickens

The dissertation thesis for the scientific degree of the candidate of veterinary sciences, speciality 16.00.03 - veterinary microbiology, epizootology, infectious diseases and immunology. National Scientific Center 'Institute of Experimental and Clinical Veterinary Medicine'. Kharkiv, 2016. The thesis is devoted to the analysis of the epizootic situation concerning reovirus infection in different poultry species (chickens, broiler chicks, ostriches, geese, Muscovy ducks) in Ukrainian poultry farms. Sequencing and phylogenetic analysis of reovirus genomes, studying the biological properties of the isolates were conducted; based on this data a reovirus strain for the construction of the inactivated emulsion vaccine against avireovirus infection (tenosynovitis) of chickens was selected. With epizootic, serological and virological investigations it was demonstrated the circulation of avireovirus causative agent among poultry in 7 regions of Ukraine. The serological researches of poultry were conducted during the period of 2006-2014. Four field reovirus isolates were selected and their biological properties were studied (pathogenicity, biological activity, the ability to replicate in cell cultures). Technological parameters of manufacturing of inactivated emulsion vaccine against chicken avireovirus from the domestic Br-06 strain were developed, also the peculiar properties of the formation, dynamics, level and duration of specific immunity in vaccinated birds were studied in the lab and high antiepizootic efficiency of this preparation in production was determined. The possibility of using 'Inactivated emulsion vaccine against chicken avireovirus from the strain Br-06' for the immunization of parental chicken flocks was proved. Key words: reovirus infection (tenosynovitis) of chickens, epizootology, biological properties of reovirus, specific prevention.