Tabachniuk N. Lectin-histochemical characteristics of submandibular salivary gland in the prenatal period of human ontogenesis

Object of the study - embryogenesis peculiarities and age anatomy of large salivary glands in man; objective - to determine lectin-histochemical patterns and developmental dynamics of topographical-anatomical relationships of submandibular salivary gland (SMSG) of man in the prenatal period of ontogenesis; methods - general-histological, cyto- and lectin-histochemical, macroscopic, microscopic, plastic and computed 3-D reconstruction, biometric, statistic; results - by means of a set of morphological methods (cyto-, histo- and lectin-histochemistry, biometrics and statistical analysis) patterns of morphogenesis and chronological sequence of SMSG formation were explored for the first time; as well as its space-time relationship during the prenatal period of human ontogenesis, variant and age anatomic variability, periods of intense and retarded growth, that made it possible to obtain new scientific evidence-based data that substantially complement current ideas about the ontogenetic chronology of human embryonic development; their arrangement was first described and the effect of glycopolymers consistent redistribution was proved - lectin receptors in the cells, on their surface and in the extracellular tissue structures during organ differentiation of epithelial and mesenchymal SMSG rudiments, the involvement of these molecules in the epithelial-mesenchymal interactions that depend on their heterogeneous origin; biosynthesis sequence and complexes activity of polysaccharide nature were studied and their role in the differentiation rate and structural transformations of SMSG and oral cavity with its derivatives in early prenatal ontogenesis was confirmed; comparative comprehensive approach to the issue of SMSG epithelium origin was first applied, that enabled to confirm the tissue nature of SMSG epithelium as ectodermal; karyometric analysis data with multi-dimensional statistical processing are new and were not used for analysis of embryonic histogenesis of SMSG before.