Lachtadyr T. Structural changes in renal cortex animals in experimental skin burn injury and under the conditions of use of infusion solutions

Using light and electron microscopy and morphometry followed by statistical processing obtained indices for the first time established the structural features of damages and compensatory-adaptive changes in the cortex of the kidneys of rats in the later stages after experimental burn of skin II-III degree, provided the use of an intravenous infusion of isotonic NaCl solution and hyperosmolar solutions (Lactoproteinum with sorbitol and Gecoton). Given the infusion of isotonic NaCl solution there mainly necrotic changes in cells that are accompanied by the development of interstitial edema, hemorrhage appearance and lymphocytic infiltrates. Given infusion Lactoproteinum with sorbitol and Gecoton spread destructive changes in renal cortex of rats is inhibited burned and substantially altered both in time and in spatial dimensions. Proved that the infusion of hyperosmolar solutions applied braking provides necrotic cells, thus inhibits the inflammatory response and promotes limited, local nature of necrotic and apoptotic changes. It was found that in rats without burn of the skin occurs mitoptosis of isolated mitochondria in epithelial cells of nephrons. This mitoptosis can be defined as basic (unstimulated) mechanism of intracellular quality control structures by removing old and defective mitochondria. For the first time described a massive mitoptosis in epitheliocytes of tubules of nephrons in burned rats, which are a form of response to factors of burn disease on cells; a mitoptosis can be defined as induced. Manifestations of its morphological differences are: 1) the large number of structurally distorted condensed mitochondria; 2) accumulation of autophagosoma; 3) association with a transparent vacuoles containing a large number heteromorphous of autophagosoma containing varying degrees of structural degradation. For the first time found that the stability of the size and distribution of mitochondria in the cytoplasm of epithelial cells in tubules of nephrons of burned rats is impaired. Mitochondria are subject mitoptosis; the formation of new mitochondria by kidney formation and separation; mitochondria fuse with one another, forming a separate and integrated into the network giant branched "mitochondrial complexes". This process is dualistic meaning: on the one hand - this is a manifestation of compensatory-adaptive reactions thereby increasing the resistance of mitochondria; on the other - this leads to distortion and extinction primary-side folded rim that has a significant impact on the state of reabsorption. It is proved that (unlike isotonic NaCl solution) Lactoproteinum with sorbitol and Gecoton do specific cell protective effect on structure in renal cortex of burned rats, thus showing nefroprotectoric properties. Specifically, Lactoproteinum with sorbitol peculiar is newly ultrastructural demonstration effect of strengthening strukturalisation of mitochondria in epithelial cells by increasing the thickness and density of electronic components all of the mitochondrial membrane. Strengthening of the mitochondrial membrane in some mitochondria is a regulator and protector of massive mitoptosis. The maximum effect is that most of the mitochondria within 14 days after the burn and gradually disappears, covering all of the smaller mitochondria, 21 days and 30 days after the burn (as the improvement in structural changes in the renal cortex and overall clinical condition of burned rats). Thus, it is a structural marker expression and "tense situation" in the cell, as well as a testimony to "improve this situation" (in this case, Lactoproteinum with sorbitol reveals his first-footed properties of the mitochohdrial tread).