Zabenko Y. Morphofunctional changes in brain of mice after repetitive mild traumatic brain injury and their correction with corvitine.

The thesis is devoted to determining of morphological and functional characteristics of mouse brain in the model of repetitive mild traumatic brain injury, as well as of effect of antioxidant drug corvitin on them, which is a water-soluble form of bioflavonoid quercetin. When conducting behavioral tests in mice after injury, the increase of rest duration and anxiety level were observed in comparison with the control. According to the results of morphological analysis of hippocampal CA1-zone, injury application was accompanied with astro- and microglisosis development. Additionally, in samples stained with methylene blue, the increase of “dark” pyramidal neurons number occurred, and electronmicroscopic samples contained electrondense neurons which indicates their possible formation on death path. The injection of corvitine led to inhibition of gliosis processes. Thus, the work shows consequences of repetitive mild traumatic brain injury even at short intervals after its reproduction, as well as anti-inflammatory and neuroprotective potential of corvitin in this kind of injury.