Yaremko O. Immunophysiological status of an organism and intensity of development of calves of milky period of cultivation under the action of pyridoxine hydrochloride

Object of research: physiological and biochemical processes in the body, immune status and indicators of growth and development of calves of the dairy period of rearing under the action of pyridoxine hydrochloride. Purpose of the work: to find out the peculiarities of the formation of immunophysiological status, growth and development of calves in the dairy period of rearing under the action of pyridoxine hydrochloride. Research methods: physiological (study of hematological parameters); immunological (determination of the number of lymphocytes, bactericidal and lysozyme activity of blood serum, phagocytic activity of neutrophils); biochemical (determination of protein content and its fractions and enzyme activity); physico-chemical (determination of the chemical composition of colostrum and cow's milk and vitamin B6 in milk and blood); zootechnical (determination of the intensity of growth and development of calves); statistical (determination of average values and their errors, level of probability). Scientific novelty: For the first time a comprehensive study of the formation of physiological and immunological parameters of calves from the 1st to the 90th day under the action of pyridoxine hydrochloride. It provides the activity of humoral factors of nonspecific resistance on the 60th and 90th days of life of calves, and cellular from the 21st day of the experiment. Pyridoxine hydrochloride causes an increase in the number of T-lymphocytes against the background of a decrease in B-lymphocytes. Feeding calves pyridoxine hydrochloride with colostrum and milk increases erythrocytopoiesis and hemoglobin content from the 21st to the 90th day of life. The increase in the number of erythrocytes was mainly due to the population of "young" erythrocytes, which contributed to the improvement of oxygen transport function of blood in calves of this rearing period and their physiological condition in general. The increase in body weight and the probable increase in absolute gains of calves depends on the dose of pyridoxine hydrochloride and is most pronounced at doses of 3 mg / kg body weight from 21 to 90 days of age. Proposed: Comprehensive studies of the formation of immunophysiological status, growth and development of calves in the lactation period under the action of pyridoxine hydrochloride. Pyridoxine hydrochloride is recommended for use in calves for 60 days of postnatal ontogeny at a dose of 3 mg / kg body weight. Implemented: Scientifically substantiated and proven the effectiveness of pyridoxine hydrochloride in dairy calves and implemented in practice in livestock farms of Ternopil region PPAF "Medobory" Ternopil region and Lviv region PPAF "Ukraine" and FE "Bogdanovych KBO". The obtained data of experimental researches on application of pyridoxine hydrochloride to calves of the dairy period of cultivation expand and deepen the available data on its influence on physiological, immunological and economic indicators, and also can be used for physiological substantiation of new ways of conducting dairy cattle breeding and improvement of existing technologies. postnatal development. The main provisions of the dissertation are introduced into the educational process and research work of the departments of Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Sumy National Agrarian University, Poltava State Agrarian Academy, Kharkiv State Zooveterinary Academy, Dnipro National Agroeconomic University, Bila Tserkva National Agrarian University, Podilsk State Agrarian Technical University, National University of Life and Environmental Sciences of Ukraine.