Object of the research: the condition of the dog’s prostate gland with chronic prostatitis and pathogenetic substantiation of their treatment regimens.
The aim of the research: clinical and pathogenetic substantiation for complex treatment of dogs with chronic prostatitis using cord blood serum.
Research methods: clinical (examination, palpation, rectal examination), sonographic, radiological, cytological (ejaculate cells), hematological (determination of hemoglobin, ESR, counting the number of erythrocytes, platelets, leukocytes, leukogram), biochemical, determination of total activity of AST, ALT, alkaline phosphatase, amylase, glucose, creatinine, urea, bilirubin), hemostasiological (determination of fibrinogen, soluble fibrin-monomer complexes), immunoenzymometric ( level of interleukin 1, 4 and factor of tumor necrosis) microbiological (generic assignment, antibiogram), statistical.
Scientific novelty: On the basis of clinical and experimental researches the pathogenetically substantiated method of treatment for dogs with chronic prostatitis using the heterogeneous cord blood serum was developed. Its high clinical efficacy has been proven, which is a significant contribution to solving the problem of restoring the reproductive qualities and fertility of dogs in case of prostate pathology. The complex approach to the diagnosis of chronic prostatitis in dogs based on clinical signs, sonographic, radiological, biochemical, hematological studies and the study of macro- and microscopic indicators of ejaculate has been improved and substantiated. The idea of expression of proinflammatory cytokines in in dogs with chronic prostatitis has been expanded. The use of enrofloxacin and cord blood serum in the treatment of dogs with chronic prostatitis as a drug with anti-inflammatory, immunostimulatory and immunomodulatory properties, increasing the permeability of the hematoprostatic barrier to antibacterial drugs and increase of antibiotic tissue deposition has been proven.
Offered: the treatment of dogs with chronic prostatitis by method with application of intramuscular injections of 5% of solution of enrofloxacin in a complex with intramuscular administration of heterogeneous cord blood serum that allows to reduce terms of treatment, promotes acceleration of leukocytes in blood recovery, decreases EST, increasing the concentration of hemoglobin, restoring the enzymatic activity of blood plasma and provides correction of fibrinogen metabolism by blocking the formation of fibrin-monomer complexes, reducing the synthesis of pro-inflammatory cytokines.
Implemented: Scientific developments are implemented in clinics of Sumy.
The expediency of ultrasound examination of the prostate by transabdominal method is substantiated, if necessary it should be supplemented by radiography together with determination of spermogram and blood tests with determination the ESR level, in addition to determination the leukocytes content. It has been determined that in order to monitor the course of the inflammatory reaction in dog’s prostatitis and evaluate the effectiveness of treatment, it is advisable to determine plasma blood activity of amylase, fibrinogen and soluble fibrin-monomer complexes, and serum concentrations of interleukins 1, 4 and tumor necrosis factor as markers of inflammation intensity. The dissertation materials are used in scientific research, practical activities and introduced into the educational process of higher education institutions in the course "Veterinary Obstetrics and Gynecology", "Veterinary Obstetrics, Gynecology and Biotechnology of Animal Reproduction with Basics of Andrology" at Sumy National Agrarian University. State Zooveterinary Academy, Poltava State Agrarian Academy, Bila Tserkva National Agrarian University, National University of Polissya.
