Zhukovsky D. Morphogenesis, function and methods of adaptation of the artificial bladder (experimental study)

The experiment showed that morphological changes in the wall of the small bowel graft occur over several months and lead to the approximation of the histological structure of the layers of the neocyst wall to the structure of the autotopic urine bladder (AUB). The described morphological changes are accompanied by functional ones. The results of cystotonometry show that, depending on the duration of the study and the experimental compound used, the MCE is from 70 to 150 cm3, and the MDT is 7.36–38.25 cm water Art. The described changes were accompanied by a decrease in the content of adenyl nucleotides, which can be considered a manifestation of an imbalance in the ATP-ADP-AMP system as a consequence of tissue ischemia and depletion of macroerg reserves used to ensure contractile activity of the detrusor. It was found that phenyl 2,2-dimethylhydrazinecarboxylate (PDHC) and N '- (4- (dimethylamino) benzylidene) benzhydrazide (DBBh), which at a concentration of 10-3 M increase the contractile activity by (53 ± 5) and (54 ± 4) %, respectively, the amplitude of contractions – by (59 ± 12) and (49 ± 14) %, the frequency of contractions – by (48 ± 6) and (31 ± 5) %, respectively. Antispasmodic properties with tropism to the ileum can be distinguished in two substances: 3- (dimethylamino) -phenyl 2,2-dimethylhydrazinecarboxylate (DPDHC) and 3- (dimethylamino) -N '- (4- (dimethylamino) benzylidene) benzhydrazide (DDBBh), which reduce hypertension by (50 ± 4) and (40 ± 3) % respectively. The maximum cystotonometric capacity was from 70 to 150 cm3, and the maximum detrusor pressure was from 10 to 52 cm of water. Art. The maximum detrusor capacity was at the level of (120±12) cm3, and the maximum detrusor pressure reached (38.25±2.14) cm water Art. Theoretical bases of possible pharmacological influence on the formation of neoplader are developed, based on the stage of its adaptation, functional state of muscles, epithelium and features of influence of original chemical compounds used for correction of contractile function. It is recommended to continue the development of chemical compounds for possible implementation in clinical practice of the results of further preclinical studies of their safety profile.