Matkovs'ka S. "Diagnosis of bovine infectious rhynotrachitis-pustulous vulvovaginitis (IBR-IPV) by antibody indices in nasal, vaginal secretion and tear.

The material for the research: The nasal, vaginsl, lacrimal secretion, the cells of the nasal and vaginal mucosal membranes. The rasearch methods: The serological, immunological, biochemical, cytomorphological,morphometric ( apparatus for electrophoresis, okular-micrometer MOV-1-1,5, microacope "Biolam-212", microreader MFNE-1U4.2. Theoretical and practical results: The possibility of using the secretion of the organism by using the Indirect Hemagglutination and Indirect Bacterioagglutination test. The diagnostic meaning of the comparison of the antibody levels in the nasal secretion and the blood serum. It has been established that in the reproduction process in the cell culture, as well as in the animal organism , IRT-IPV virus form Basolilie intranuclear inclusion, which later aguire Oxyphlie properties. It has also been proved, that by using cytomorphological method. It is possible to determine the effeciency of the intranasal immunisation with live vaccine. The practical use of the research: The elaborated methodical recomendation about the use of the secretions of the organism for IBR-IPV diagnosis by Indirect Hemagglutination and Indirect Bacterial Agglutination test, that have been approved by the Adriculture and foodstuffs of Ukraine are now already bein used in the academic programme of the National Agrarian Universitydepstment of Epizootology and the Kharkov Zooveterinart Institute - department of microbiology, virusology and immunology; they have also been put into practice at the Regional Veterinary laboratory of Lugansk.