Bila N. The development of the method of infectious laryngotracheitis and Newcastle disease diagnosis by means of fluorescent probe

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0499U001880

Applicant for

Specialization

  • 16.00.03 - Ветеринарна мікробіологія та вірусологія

22-06-1999

Specialized Academic Board

Д 64.359.01

National Scientific Center Institute of Experimental and Clinical Medicine

Essay

The object of studying: pathological mathe-rial; blood serum; infectious laryngotracheitis viruses; Newcastle disease viruses; fluorescent probe. The purpose: to develop the criteia of diagnosis efficiency of fluorescent probes method for identification of infectious laryngotracheitis and Newcastle disease agents in poultry and to carry the comparative charactevization of to compare proposed method with existing ones. The methods of investigations: virusological, serological, statistical. Equipement: luminiscent microscope with photometrical nozzle, centrifuge, termostate. The theoretisal and practice results: it has been proposed the method of fluorescent probe using for infectious laryngotracheitis and Newcastle diagnosis. The established temperature de-pendence of VSIIHP-U strain is entered to the technological terms of live virus-vaccine against ILT making. Novelty: in first the fluorescence probes method has been developed for ILT and Newcastle disease diadnosis. Dynamics and peculiarities of ILT and NCD accumulation under condition on chiken embryos has been studied. The degree of incultation: it has been developed the project of "Temporary directions on ILT and NCD diagnosis by means of fluorescent probes". The bield of use: poultry farming.

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