Klestova Z. Theoretical and experimental substantiation of the cell genome's control in the course of animal viral infections and in the process of the development of means of their specific prophylaxis

Українська версія

Thesis for the degree of Doctor of Science (DSc)

State registration number

0506U000429

Thesis Registration Form

0506U000429.pdf

Applicant for

Specialization

  • 16.00.03 - Ветеринарна мікробіологія та вірусологія

29-06-2006

Specialized Academic Board

Д 26.004.03

National University of Life and Environmental Sciences of Ukraine

Essay

The thesis is devoted to the problems of revealing the influence of pathogenic animal RNA - viruses (members of the families Reoviridae, Picornaviridae, Coronaviridae, Retroviridae, Flaviviridae) on the genome of infected cells: the determination of the features of their interaction with sensitive biological systems (animal cells and organisms); the use of these features for indication and directed alterations of biological properties of causative agents in the development and creation of new means of specific prophylaxis of viral diseases in animals. The presence and circulation among animals at specialized farms and units of these pathogens was established; their isolation, identification and adaptation in biological sensitive systems of cultivation were carried out (according to the determined optimal methods and conditions). The damage of microvilli of intestinal enterocytes in piglets was revealed under the influence of swine rota- and coronavirus before their adsorption on cell membrane.Mutageniceffect of members of five families of RNA - containing animal viruses was revealed. This effect was enhanced by combined influence of the pathogen on the genom of infected cells. A test-system for the detection of antimutagens was developed and the possibility of viruses' mutagenic activity alterations by means of preparations produced from plant raw materials was investigated.Inactivating effect of 0,01 % chloroform solution on the classical swine fever virus determined. Damages of the structure of swine rotavirions were identified under the influence of ultrasound power of 500 W, frequency 22 kHz during 10-20 sec., which causes the damage of outer capsid of the virus. Heterogeneity of swine rotavirus population and the alteration it's structure in process of pathogen adaptation to a sensitive biological system were determined. A stable micropopulation of the causative agent was identified. Vaccines against rotaviral disease and classical swine fever were developed and produced.

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