Gerilovych A. Experimental and theoretical study and development of epizootological monitoring, diagnostics of animal viral diseases and their agents molecular-genetic typing means (orthomyxo-, paramyxo-, herpes-, circo- and pestyviral infections).

The unified system of primer sequence calculating was elaborated based on search of target genes, analysis of their conservative sites, primer bioinformatics detection and assessment of its quality in theoretical micro- and mаcroanalysis. The effectiveness of the system proved to model RNA and DNA viruses of animals matrices of five viral families, so developed two vector systems, nine methods of detection, identification and genotyping avian influenza virus (type A, subtype H5, N1), Newcastle disease virus, infectious bovine rhinotracheitis virus, Aujeszky disease virus, infectious laryngotracheitis virus, porcine circovirus and bovine viral diarrhea virus. Methods for vector system construction and pathogen detection based on PCR were patented in Ukraine for utility models. The first time the molecular and phylogenetic studies epizootological Ukrainian avian influenza virus isolates (2005-2008), porcine circovirus (2006-2008), Newcastle disease virus (1967-2008) and bovine diarrhea virus (2006-2008) was done. On the basis of this study the genotypes of pathogens and their relationship with other genetically similar populations were defined. Several hypotheses regarding possible sources of ingress and modifying them in populations of farm animals and poultry in Ukraine were formulated. By the results of researches, six molecular genetic test systems for detection of DNA and RNA viruses of animals were tested in interlaboratory trials and registered in Ukraine. Scientific-Methodical Council of State Committee of Veterinary Medicine of Ukraine adopted "Guidelines on the calculation praymernyh systems for detection and identification of pathogens of animals" and 6 recommendations for diagnostic protocols.