Brezvyn O. Mycotoxin control and their neutralization

This thesis justifies theoretically toxicological control of mycotoxins in feeds and their neutralization. Methodical questions are highlighted concerning determination methods of mycotoxins in feeds and practical application of modern correction means of disintoxicating systems of animal organism at mycotoxicosis. Monitoring and systematization of received test results of feed raw material from western region of Ukraine showed that 35 %-40 % of corn feeds are affected by potentially dangerous microscopic fungi, population of which is presented by Fusarium, Alternaria, Rhizopus, Mucor, Aspergillus and Penicillium. T-2 toxin, DON, zearalenone, fumonisin, aflatoxin and ochratoxin were also detected. New approach to fumonisin B analysis in feed by using TLC and HPLC that allows to detect and identify them qualitatively and quantitatively, to conduct mycotoxicological control. On the basis of analysis of literature sources concerning fumonisin toxicity and tests national fumonisin MRL in compound feeds and feeds for animals - 5 mg/kg and in compound feeds for fish - 10 mg/kg at feed humidity 12 %. The system of mycotoxicological feed and feed additive control was firstly developed on experimental prototype of biosensor device. New express method of feed control was proposed that allows selectively determine qualitative and quantitative mycotoxin contents in feed raw material. Complex pharmacotoxicological and experimental inspection of proposed means was conducted - alfasorb and high-sodium hypochlorite solution at experimental mycotoxicosis of rats, hens and piglets.