Suprovich T. Molecular-genetic analysis major histocompatibility complex in connection with stability and receptivity to mastitis for cows

In this thesis research proved the concept of complex application of molecular genetic markers for effective early diagnosis of susceptibility and resistance to mastitis cows Ukrainian black-pied dairy breed and Ukrainian red-pied dairy breed based on antigens class 1 major histocompatibility complex alleles and exon 2 gene DRB3 BoLA-system. The complex molecular-genetic analysis of alleles gene of BoLA-DRB3 (class of II) is carried out in connection with receptivity and resistance to mastitis for the cows of Ukrainian black-pied dairy breed and Ukrainian red-pied dairy breed. For the cows of black-pied breed receptivity to mastitis is alleles BoLA-DRB3.2*24 and *26. Alleles BoLA-DRB3.2*13 and *22 specify on an association with resistance of cows to mastitis. In populations of cows of the Ukrainian red-pied dairy breed with receptivity to the diseases of udder associated alleles gene of BoLA-DRB3.2*07 and *08. Alleles *22 and *24 have close connection with resistance to mastitis. To two domestic breeds of cattle on the basis of analysis of antigens of BoLA-A the worked out method of individual integral estimation of immunogenetic status of animals (by the method of statusmetriya), which allows to control the risk of morbidity on mastitis for every individual.