Kovalchuk O. Morphological features of rats adenohypophysis with experimental skin burn injury and the conditions of use of combined infusion of hyperosmolar solutions

The thesis is devoted to the determination of signs of structural changes in rats adenohypophysis after thermal skin burn and in the case of the use of combined hyperosmolar solutions (Lactoprotein with sorbitol and HAES-LX-5%). The comparative analysis of the effect of 0.9 % NaCl solution and Lactoprotein with sorbitol and HAES-LX-5% demonstrates objective benefits of cytoprotective effects of the last in the case of the studied correction of the skin thermal burn outcomes. Intravenous infusion of Lactoprotein with sorbitol and HAES-LX-5% to rats with experimental second and third degree skin burns (21-23 % of the total body surface area) is associated with the decrease of endogenous intoxication indices, with positive effects on macroscopic, histological, ultrastructural and morphometric aspects of compensatory and adaptive responses and optimizes cell cycle indices (SUB-G0G1, S-phase, G2+М phase) of DNA fragmentation (SUB-G0G1 interval) in rats adenohypophysis after 1, 3, 7, 14, 21 and 30 days of observation. The results of the dissertation revealed peculiarities of the positive impact of Lactoprotein with sorbitol and HAES-LX-5% on morphological state of adenohypophysis after experimental skin burn injury. The abovementioned results may become the theoretical basis for the development of cytoprotective method of correcting negative effects of the severe thermal injury.