Hrushanska N. Theoretical and experimental substantiation of non-invasive methods for the diagnosis of metabolism disorders of the trace elements in cattle and pigs

In dissertation work the results of complex diagnostics of disorders of mineral metabolism in the cows and sows of the central and north-eastern biogeochemical zones of Ukraine with the use of non-invasive methods are presented. It is proved that the application of the developed OK-1 device, methods and equipment for determining the biophysical parameters (clinical parameters) of the body of the cows (body temperature, respiratory rate, heart rate and rumen) indicates that there is no significant difference in these indices in the animals of the central and north-eastern biogeochemical zones, in comparison with the use of classical methods of diagnostics, which saves time of the researcher 1.8–2 times and provides the opportunity to standardize these parameters with the accumulation of data array in electronic bases. The content of copper, iron, zinc, cobalt, aluminium, chrome and nickel in the saliva of cows and sows during lactation was investigated and methods of selection of non-invasive biological substrates for multi-element analysis were improved. The concentration of copper, iron, zinc, cobalt, aluminum, chrome and nickel in the hair cover of cows and sows in the north-eastern and central biogeochemical zones of Ukraine was determined. The application of the biologically active additive «Oxymnikor», which includes copper, zinc and cobalt lactate, succinic acid, sodium salt of humic acids and glauconite for prevention of microelementosis in cows during the course of 28 days significantly increases hemoglobin content in the blood by 16 %, copper in 1,3 times, zinc in 1,4 times and the number of erythrocytes by 12,8 %. The use of biologically active additive «Suilactomin-oxy», which consists of copper, zinc and cobalt lactate, succinic acid, sodium salt of humic acids and glauconite for the prevention of microelementosis in sows, significantly increases cobalt content by 4,5 times, copper in 1,1 times, zinc in 1,4 times, and in saliva it increases the content of iron in 1,4 times.