Vyhovska L. Biological properties of food zoonotic agents and their indication tools development

In the dissertation we studied biological properties of bacterial food-borne zoonoses. The species characteristics of sensitivity to antibacterial preparations Salmonella spp., Listeria spp., Yersinia spp., R. multocida, Campylobacter spp. have been characterized. We described the changes in culture-morphological, enzymatic, antigenic, pathogenic properties, sensitivity to antibacterial preparations for various types of modifications Salmonella spp., Listeria spp., Y. enterocolitica, Y. pseudotuberculosis, P. multocida, Campylobacter spp. We characterized biological properties of Salmonella spp and E. coli isolates with acquired multiple resistance to antibacterial drugs. We found that 100 % of investigated isolates are resistant to nitrofuran, to which drug resistance is rarely developed. There is established a coincidence of the sensitivity level of Salmonella spp. and E. coli obtained from the same object to certain antibiotics. The necessity of improving the laboratory diagnosis of bacterial zoonoses by conducting complex studies using microbiological and molecular genetic methods was experimentally grounded. There is established that the influence of aurum and bismuth nanoparticles on of bacteria growth properties depends on the size of nanoparticles, their concentration in the medium and biological properties of the microorganism. The results of our research became the scientific basis for the development of diagnostic tools: antigens set for animal salmonellosis diagnosis, a diagnostic kit for the bacterial DNA detection of the species Yersinia enterocolitica by the polymerase chain reaction «Yersinia enterocolitica – PCR test», a diagnostic kit for the bacterial DNA detection of the species Campylobacter spp. the method of polymerase chain reaction «Campylobacter spp. – PCR Test», diagnostic kit for the bacterial DNA detection of Listeria bacteria by polymerase chain reaction «Listeria spp.» – PCR Test, test strains set of Listeria spp. for conducting CAMP-test and quality control of nutrient media, as well as methods for indicating «Salmonella spp. real-time polymerase chain reaction in real time; and identification of Shiga toxin-producing (stx1/stx2) Escherichia coli by polymerase chain reaction in real time.»