Strus O. Theoretical and experimental substantiation of complex use of sapropels for creating medical, veterinary and cosmetic remedies

Українська версія

Thesis for the degree of Doctor of Science (DSc)

State registration number

0521U101205

Applicant for

Specialization

  • 15.00.01 - Технологія ліків, організація фармацевтичної справи та судова фармація

11-05-2021

Specialized Academic Board

Д 35.600.02

Danylo Halytsky Lviv National Medical University

Essay

The objects – sapropel, dry sapropel, sapropasta, oil sapropel extract (OSE), aqueous sapropel extract (AqSE), alcohol sapropel extract (AES), soap with sapropasta, cream with OSE and AqSE, gel with AqSE; excipients and comparator products; the purpose - the aim of the dissertation is to create a scientific and methodological approach to the complex use of sapropel, development and standardization of promising medical, veterinary and cosmetic remedies with sapropel processing products; methods - general scientific (analysis and generalization), organoleptic (color, odor, homogeneity, etc.), chemical (Kjeldahl’s titrimetric method, intermediate filtration method), physical, physico-chemical (gravimetry, potentiometry, photocolorimetry, chromatography mass spectroscopy, capillary electrophoresis, gas-liquid chromatography); as well as methods for assessing the structural and mechanical properties of semi-solid dosage forms (structural viscosity, spreadability, extrusion capacity of semi-solid medical and cosmetic remedies based on native sapropel and sapropasta); pharmaco-technological methods of researching quality indicators of the developed drugs (description, identification, homogeneity, weight of container contents, etc.); microbiological (antimicrobial activity, effectiveness of preservatives, microbiological purity); pharmacological (determination of pharmacological activity and toxicity of sapropel and preparations based on it); research methods of quality indicators of the developed preparations; statistical; results - the complex use concept of sapropel is substantiated, the general research methodology is developed; technology is worked out, researched and standardized sapropasta; the composition and technology of antibacterial soap with sapropasta are confirmed; substantiated the technology of aqueous sapropel extract using the dispersion method, which increases the yield of humic acids, compared with the classical method, more than 9%; substantiated and developed the technology of oil sapropel extract using step two-phase extraction by 90% ethanol and corn oil, which provides an increase in chlorophyll yield by 24% compared to single-phase extraction; the composition is proved, the technology is developed and the quality indicators of semi-solid dosage forms under the conditional names: "Saprogel" and "Saprocream" are investigated; methods of identification and content quantitative determination of the total mass fraction of humic acids in the aqueous sapropel extract, gels and creams with sapropel extracts are developed; quantitative determination methods of the amount of natural pigments and chlorophylls in oil sapropel extract, cream with sapropel extracts; validation of the developed methods is carried out; the anti-inflammatory and wound-healing effect of “Saprogel” in the second phase of the wound process, as well as its antioxidant properties were established on the model of full-thickness wounds in rats; on the model of UV erythema in rats is showed a pronounced anti-inflammatory activity of soft dosage forms with sapropel extracts, which is most pronounced in "Saprogel"; with skin application of aqueous, alcohol and oil extracts of sapropel; sapropasta, preparations: "Saprogel" and "Saprocream" detected no toxic effect, sensitizing and local irritant properties; clinical trials of Saprocream have proven its effectiveness and safety in the treatment of microtraumas, erosions and cracks (wounds) of teats on cow’s udders; implemented - TSU 20.1-2870305538 - 004: 2016 "Natural cosmetic sapropels and their products" for sapropel, sapropasta and AqSE is developed and approved; the projects of the technical specifications(TS) and quality control methods (QCM) on the production of "Sapropasta", AqSE, OSE, preparations "Saprogel", "Saprocream" and foaming antibacterial agent with sapropasta are developed; the technology of pharmaceutical developments has been implemented in the industrial condition on the pharmaceutical factory "Chervona zirka", Ukraine (Kharkiv), LLC "Zender-Ukraine" (Ukraine, Lutsk), RPPhC "AIM" (Ukraine, Kharkiv), AS "Pharmaco" JSC (Republic of Moldova, Chisinau); in the scientific and educational process of pharmaceutical (medical) institutions of higher education and research institutes of Ukraine and foreign countries ; branch - pharmacy.

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