On the basis of systematic analysis of scientific approaches to researching investment and innovation maintenance of regions development, conceptual principles of innovation and investment support for region development are developed. The main components of innovation and investment support for region development in terms of functional connections that form the conceptual foundations of the research, which determine its areas, objects, regulatory framework, institutional environment, sources, principles, conditions and effects are identified and characterized. The paper reveals the prerequisites for increasing investment and innovation support in the region in terms of decentralization changes. Based on the analysis of the best domestic practices in increasing the investment and innovation support of region development, the conditions for increasing the regions’ resource base of investments in terms of decentralization are singled out. In particular, the global conditions include: the relevance of legislation on investment activities and legal liability for their violation; mutual responsibility of the subjects involved in the investment process; observance of the rights and freedoms of the investor, expansion of mixed financing forms of investment projects, etc. The main elements of investing at the local level are systematized, which include: investing by individuals / legal entities based on collective ownership; state and local investment; state support for the implementation of local projects; foreign and joint investment. The study of investment and innovation support of the region in the terms of decentralization changes allowed to form the criteria of regions investment attractiveness. In particular, they are: the territory readiness for the investment process; no problems with ownership of the object of investment; popularization of investment advantages of the territory, which are peculiar only to it; transparent legal status of the territory; demonstration of positive economic changes in the region; consistency of investments that are attracted to the community. The world experience of investment and innovation support of region development in the terms of decentralization is studied. Based on the critical analysis, the functions performed by the state in regulating investment and innovation activities are highlighted: regulatory, stimulating, administrative. The main tools for making innovative and innovative decisions in world practice are macroeconomic regulation, tax and depreciation policy. The methodical approach to carrying out diagnostics of investment and innovative maintenance of regions development on the basis of using the method of integrated index analysis is developed. There are three criteria for assessing investment support (concentration of investment resources, diversification of sources of investment, investment efficiency), and four criteria for assessing the level of innovative support for regional development (research activity of scientific organizations, innovation activity of industrial enterprises, results of innovative activity of industrial enterprises, efficiency of innovative activity of industrial enterprises). It was found that the regions of Ukraine differ significantly in the level of investment support for development. The results of diagnostics of the level of innovative support of regional development showed that only Kyiv, Zaporizhzhia, Sumy, Kharkiv regions were in the group with a relatively high level. While the most numerous is the group with a low level of innovative development, which requires the development of anti-crisis strategies for innovative development, taking into account their regional characteristics. The approach to the formation of innovation and investment strategies of regions based on a comprehensive diagnosis of levels of investment and innovation support of development was justified, which allowed to build a field of relationships between levels of investment and innovation support of regions development in Ukraine according to integrated assessments, and on this base a strategic concept of investment and innovation support of the state regions development was developed and the positioning the regions of Ukraine in the matrix of choosing the type of innovation and investment strategy was held. The paper improves the approach to building a correlation and regression model of the dependence of Volyn gross regional product on innovation and investment support, which allowed to determine the multiplier effect of the cost of innovation, the number of employees involved in scientific research, the cost of research and development, capital and foreign direct investment in the region’s economy. It is proved that the prospects of social and economic growth of the region depend on the combination of public sector potential and private capital, which is fully ensured through the implementation of public-private partnership agreements.