The thesis is devoted to deepening of theoretical and methodological provisions regarding the development of organizational culture mechanism in a multinational organization operating in conditions of diverse national cultures. The object of the study is the development process of organizational culture mechanism in a multinational organization. The subject of the research is a set of theoretical provisions, methods, methodological approaches, methodological support and practical recommendations for the development of organizational culture mechanism in a multinational organization operating in conditions of diverse national cultures. The author has defined the essence of the concept "organizational culture in a multinational organization" by using methods of theoretical generalization, analysis, synthesis, and structural-logical analysis. The work proposes a refined definition of the concept "organizational culture in a multinational organization", the novelty of which is the combination of a value approach to the essence of the concept "organizational culture" and the nature of the concept "multinational organization". There is a detailed analysis of conceptual approaches to determining the essence of organizational culture's nature at the micro level based on primary characteristics and the essence of the phenomenon "organizational culture" examined through the prism of organizational culture characteristics. The thesis systematizes and analyzes the factors of the external and internal environment affecting the processes of formation and development of the organizational culture in a multinational organization. The proposed classification of factors of the internal environment is based on the division of factors into general organizational, managerial and personnel-related factors, which allowed the author to determine the most important structural components of the organizational culture mechanism in a multinational organization. The author uses the classification of approaches to define the essence of "organizational culture in a multinational organization". The thesis generalizes the definition of the concept "mechanism of organizational culture in a multinational organization", which is the result of the synthesis of multidimensional and multilevel approaches to the study of the mechanism of organizational culture. The novelty of the proposed concept "mechanism of the organizational culture in a multinational organization" is its consideration as an organizational and economic mechanism, implemented in the management system as a set of structural elements. These elements are principles, functions, factors, influencing the development of organizational culture, resources, methods, tools and means of influence on personnel processes, used by the management of the organization at all hierarchical levels and the result of the implementation of which is the complete identification of employees with the organization. In the thesis, the author develops the mechanism of the organizational culture in a multinational organization, which allows us to adapt the key characteristics of the organizational culture in accordance with the influence of external and internal environmental factors in order to obtain the desired staff behavior to achieve the goals of the multinational organization. A methodical approach to the comprehensive assessment of the organizational culture of the staff in a multinational organization, taking into account the national content, is proposed and developed in the work. This is the result of a combination of three approaches to the assessment of culture: RVS, PVQ and 6-D model. Its novelty consists in taking into account the level of compatibility of the organizational culture's dominant type with the requirements of the national culture of the country in which the organization operates, and the possibility of adjusting its components in accordance with these requirements. The methodological basis of the research is the fundamental provisions of modern theories of economic theory, management and cross-cultural management. Practical significance of the obtained results is that the use of theoretical and methodological provisions of the thesis work has been brought to the level of specific methods and recommendations for theoretical and methodological support for the organizational culture mechanism development in a multinational organization. The Chinese multinational organization Television Culture Media Co. Ltd (sertificate dated 23.05.2023). "VENT-MEDICAL LLC" (sertificate № 11/06 dated June 26, 2023). Simon Kuznets Kharkiv National University of Economics (implementation certificate No. 23/86-02-33 dated 13.06.2023 year). Introduced into the educational process of Neijiang Pedagogical University Normal University, School of Economics and Management in Business Administration disciplines (implementation certificate dated 04/25/2023).