Kyrychenko S. Molecular mechanisms of vascular hypercontractility induced by ionizing radiation and its pharmacological correction

The dissertation is devoted to experimental investigation of the role of rat aortic smooth muscle cells (SMCs) large conductance Ca2+-activated K+ channels (BKCa) in vascular hypercontractility and hypertension induced by ionizing irradiation. The repairing effects of quercetin-filled phosphatidylcholine liposomes (PCL(Q)), free quercetin (Q) and "empty" phosphatidylcholine liposomes (PCL) on BKCa channels activity after non-fatal whole body ?-irradiation was studied. ?-Irradiation suppressed outward current in rat aortic SMCs due to BKCa channels conduction decrease. On the 30th day postirradiation pore-forming ?- and regulatory ?1- BKCa channels subunits mRNA expression levels considerably diminished. siRNA-induced BKCa deficiency in vascular wall may contribute to endothelium-dependent relaxation and vascular hypercontractility under ionized irradiation. PCL(Q), free quercetin and "empty" phosphatidylcholine liposomes (3.4 ?g/ml by quercetin, 124.0 ?g/ml by lecithin) possess the ability to normalize BKCa malfunction following irradiation.