Nefidova V. Сytogenetic characteristic in adolescents with osteoarthrosis

Object of the research: сytogenetic peculiarities and the level of mutational chromosome variability at adults with osteartrothis, before and after mutagen loading with mitomycin C, C-polymorphism geterochromatin areas of 1, 9, 16 and Y chromosomes, accumulation of rheumatic diseases in families of probands. The purpose: studying morphofunctional features of chromosomes at adults with osteartrothis. Methods of the research: genealogical, сytogenetic (classical with the use of differential C-coloring), statistical. Theoretical and practical results: The new basis and decision of a scientific problem which is regarded to researching of chromosom apparatus in adults with OA have been presented and for the first time. For the first time on the basis of studying of a condition of the chromosomal apparatus probands, the increase of spontaneous level of chromosomal infringements in 3 times in the general group of patients has been established testifies to destabilization genome at osteartrothis. The frequency of increasing aberration chromosomes at teenagers depending on sex, character of hereditary burding and duration of a current of disease has been revealed. For the first time level of mutational variability in lymphocytes of peripheral blood probands with osteartrothis after interaction of a modelling mutagen has been estimated. The increase of the general level chromosomal aberrations after additional mutagen loading at probands with osteartrothis and healthy contemporaries has been proved. The increase in the general level chromosomal aberrations after additional mutagen loading at probands with OA and healthy contemporaries is proved and also in groups of patients, osteartrothis at which it was formed on the background of a hypermobile syndrome (HMS) and jet arthritis (RhA). Features of variability C-heterochromatin areas of chromosomes has been established at osteartrothis for the first time which were shown by reduction of the sizes CHCh of chromosomes 1,9,16 at probands with osteartrothis on the background of HMS and RhA. For the first time on the basis of the conducted research informative prognostic signs for revealing of persons of high risk on infringement of the chromosomal device at probands with OA are allocated. Reliability of offered criteria of forecasting has made 68 % on a method of Valda. Use of criteria of forecasting for revealing of persons of high risk on infringement of the chromosomal device at probands with ОА will allow to carry out the timely actions promoting stabilization genome. Application field: genetics.