Plis V. Epizootological monitoring of avian pasteurellosis (fowl cholera) and improvement of diagnostics.

Materials on the study of the epizootic situation concerning avian pasteurellosis (cholera) are presented in the dissertation. Bacteriological tests to study the circulation of the pathogen in the private sector and at the farms of different forms of ownership in Ukraine with different technology of keeping, growing and of different productive directions have been carried out. For the in vivo diagnosis of avian pasteurellosis (cholera) there has been proposed the use of modern diagnostic kit. At the manufacture of erythrocyte antibody-antigen for in vivo diagnosis of avian pasteurellosis (cholera), an optimal scheme of ram erythrocyte fixation has been chosen experimentally. A study on the sensitization of treated with tannin ram erythrocytes by avian pasteurellosis (cholera) bacteria has been carried out. A study to determine the optimal scheme of immunization of poultry and animals (cocks, geese and rabbits) to obtain a specific hyperimmune blood serum to the agent of pasteurellosis has been conducted. The results of the investigated tissue homogenates (glandular stomach, thymus, spleen, liver and heart) showed that high concentrations of avian pasteurellosis (cholera) bacteria was observed in the liver (1:256) and in the heart (1:128). A comparative evaluation of the results of indirect hemagglutination test and agglutinating immunoassay with erythrocyte antibody-antigen at the detection of the level of specific antibodies to avian pasteurellosis (cholera) bacteria has been carried out. The results indicate that the quantitative reaction of indirect hemagglutination test using erythrocyte antibody-antigen makes it possible to control the epizootic situation concerning avian pasteurellosis (cholera), to control the growth of specific antibodies in blood serum, to determine the percentage of the effectiveness of immunization of poultry population. Agglutination test makes it possible to quantify only the presence of antibodies in the blood serum to avian pasteurellosis. The obtained results have been used to develop a kit for in vivo diagnosis of avian pasteurellosis (cholera) in the indirect hemagglutination test.