Golovan L. The peculiarities of different types of marker systems usage in the selection investigation of the PHASEOLUS L. genus

The object of the investigation: morphological signs, isoenzyme systems ability of different varieties and species of beans to cross, hybrids F1 productivity, form-building process of the second generation hybrids and their productivity and also RAPD-locuses. The purpose: to learn morphological and biological signs of the beans collection and to determine selection importance of the analyzed gene pool, to reveal the sources of economic valuable signs, to create new initial material on their basis and also to ascertain polymorphism of the morphological signs, isoenzyme systems, their genetic control and cohesion with locuses that code beans morphological signs, establishment of the genetic polymorphism level of RAPD-locuses of the collection beans samples for the purpose of selection process efficiency rise. The methods: field, laboratory and field, mathematical and statistical, biochemical, molecular and genetic. The novelty: the polymorphism of isoenzyme systems of different beans species was determined, biochemical passport systems and classification of samples with the help of isoenzyme were help, the level of setting of hybrid seeds with the help of isoenzyme systems through intraspecific hybridization was established. Genetic control of shikimic dyhydrogenese system was established, the group of isoenzymic locuses cohesion with morphological signs were studied. Molecular and genetic polymorphism of RAPD-locuses of bean samples was ascertained, the level of their genetic proximity was determined. The specific fragments which were used in the capacity of markers while the collection identification were revcaled. The peculiarities of changeability and inheritance of quantitative signs that intraspecific and interspecific hybrids of the fitst and the second generation have were established. The transgressive forms were detected. The peculiarities of changeability of the main economic valuable signs of the beans collection, their interconnections were determined. The perspective sources of morphological and biological signs were singled out and the new initial material for selection was created. Results: fourteen sources of valuable traits have been chosen and 10 families as the initial material of cjmmon bean. The rate of introduction: the sources were singled out and new initial material for beans selection with stable signs display was created. These signs were introduced into the selection programmes of the genetics, selection and seed growing subdepartment of Dokuchayev Kharkiv National Agrarian University and the Institute of agriculture in the Steppe Zone in NAAS. Passport system was help and identification formulas of 25 samples which are used by the National center of genetic resourses of plants in Ukraine were created. Genetic distances between the samples were held on the basis of the carried out morphological, isoenzyme and RAPD analyses that are used while choosing the paternal forms of hybridization. The sphele of introduction: selection establishments.