Mozheitova O. Role of prostaglandin F2a in the regulation of bile secretion

Object: bile formation liver function. Purpose: To study the influence of endogenous prostaglandins and exogenously introduced prostaglandin F2alfa on bile formation. Methods: physiological (acute experiments on rats), biochemical (TLC) and the methods of statistical analysis. Results: In case of depressed synthesis of endogenous prostaglandins by acetylsalicylic acid the rate of bile secretion appeared to stay without any change but the output of glycocholates was diminished. After dexamethazone injection the volume of secreted bile as well as the biliary content of taurocholates and some lipid components has been increased. Both acetylsalicylic acid and dexamethazone caused the decrease of cholate-to-cholesterol ratio and the correlation of trihydroxy bile acids to dihydroxycholates that indicates the impairment of emulsifying and solubilising properties of bile and increase of its lithogenicity. Administration of prostaglandin F2alfa results in dose-dependent decrease of the bile secretion rate and biliary output of unconjugated (free) bile acids. In response to alfa-adrenergic blockade inhibitive effects of prostaglandin F2alfa on bile formation are negated. It is also followed by the elevation of the volume of secreted bile and biliary content of taurine conjugated bile acids. Application areas: physiology, pharmacology.