Bondzyk O. The influence of L-arginine on oxygen balance and exocrine function of the liver

Object: blood flow, oxygen homeostasis, exocrine function of the rat liver. Objective: To determine the features of the influence of L-arginine on oxygen balance and exocrine function of the liver in rats and explore the possible contribution of nitrogen monoxide in the implementation of these actions. Methods: In acute experiments on rats, determination of blood pressure in the vessels using manometer, determining oxygen tension in the liver tissue and the consumption of oxygen using polarographic method, cannulation of bile duct, thin layer chromatography to determine the concentration of bile acids and lipids in the bile, statistical methods of processing the experimental data . Results: L-arginine at doses close to its physiological concentration levels in blood (5 and 10 mg/kg) activates oxygen-dependent processes of biosynthesis and biotransformation of organic components of bile (bile acids and lipids), increasing the blood supply to the liver and its tissue respiration, the level of oxygen tension in gland is not changed, reduced volume rate of secretion of bile. Amino acid 5 mg kg improves solubilization properties of bile. Only some of these effects are NO-dependent. Novelty: We show that the stimulating effect of L-arginine at a dose of 5 mg/kg, close to its physiological concentration in blood is partly involving NO. At a dose of 10 mg/kg activating effect of L-arginine on liver tissue respiration in the first 20 minutes after administration implemented without nitrogen monoxide, but further activating effect of L-arginine is with NO. L-Arginine reduces the volume of bile secreted and affects the conjugation of bile acids and their transport in bile. Not all effects of L-arginine realized through its ability to be a precursor to the synthesis of NO. Scope: human and animal physiology, medicine, pharmacology.