Prysiazhniuk L. Peculiarities of expression and evaluation methods of genetic constructs in transgenic plants of sugar beet

The property features research organization and display of transgenes in plants of sugar beet. The purpose of research - installation of display features and how to assess genetic structures in transgenic sugar beet plants, research ways of expression in stable and components hybrids and developing a system of diagnosis and assessment of transgene expression in the genome of sugar beet. Research methods - conventional. The thesis presents the results of research to develop a method for assessing the activity of genes by polymerase chain reaction in conjunction with reverse transcription and multiplex PCR to identify the sequences of 35S promoter and als EPSPs genes in plants sugar beet tolerant to glyphosate action. Expediency of total RNA extraction from phenol and guanidine thiocyanate temperature incubation in step lysis + 60 ° C and Oligo (dT) -primer for reverse transcription reaction to determine gene activity EPSPs. Established close relationship of the gene of interest and promoter sequences for their detection in sugar beet genome and the gene of interest activity dependent on the presence of 35S promoter sequence and gene EPSPs. It is shown that the purpose of selecting breeding material tolerant to glyphosate action, appropriate priority to identify regulatory sequences and a gene of interest, followed by determination of its activity. According to the research developed guidelines and implemented in the educational process of the National University of Life and Environmental Sciences of Ukraine for training in specialties "Biotechnology" and "Breeding and genetics of crops."