Ushkalov A. Biological properties of Yersinia and development of their means of detection

The thesis is aimed at expanding scientific knowledge on the spread of Yersinia, their etiological role in food zoonoses and study the biological properties of Yersinia spp. to improve the means of indication Yersinia enterocolitica. The thesis shows the data on the current system of international supervision (under the auspices of WHO) for zoonoses food chain transfer, the place Yersinia spp. as etiological factor of food zoonoses, presented data about Yersinia spread in the world and Ukraine, as well as sources and factors of transmission, which became the theoretical basis for developing means of express indication Yersinia enterocolitica DNA molecular genetic techniques and the creation of a collection of strains of Yersinia spp. Results the study of cultural-morphological, enzymatic properties of pathogenicity for laboratory animals and antibiotic resistance rise to 26 strains of deposit Y.enterocolitica, 5 - Y.kristensenii, 38 - Y.rseudotuberculosis and create a collection of strains at the National Center of microorganisms SSCIBSM. The possibility to use aerosil A-300 as a part of a protective environment for lyophilization and storage Yersinia, use one provided significant increase preservation studied microorganisms to 7.6% after 12 months of storage as compared to medium-prototype. Experimentally proven efficiency of the means of molecular genetic indication Yersinia enterocolitica which was the basis for his registration in Ukraine. Key words: yersiniosis pathogens zoonotic diseases food source of the pathogen, biological properties, pathogenicity, antibiotic resistance, polymerase chain reaction, specificity, sensitivity.