Zhylkova I. Genetic aspects of low reproductive function in men of Eastern Ukraine

Genetic features of men with reduced fertility in the Eastern region of Ukraine have been studied. Objective: to determine the molecular genetic and cytogenetic factors of spermatogenesis, leading to impaired fertility in men and, as a consequence, affecting the early development of embryos obtained from such patients using assisted reproductive technologies. Methods: differential staining of chromosomes with GTG and CBG methods, fluorescence in situ hybridization, PCR, real-time PCR, enzyme immunoassay, morphometry, microscopic studies, statistical analysis. Novelty: the association of an increased level of DNA fragmentation in male spermatozoa with a decrease in fertility with the polymorphic variants G919A and A2039G of the FSHR gene is presented for the first time; for the first time it was showed that the level of genetic disorders in spermatozoa in men in Eastern Ukraine increases after 35 years; new criterion for a normal level of DNA fragmentation in spermatozoa - less than 20%; the critical level of DNA fragmentation in spermatozoa for the blastocyst formation rates for the population of Eastern Ukraine is established; the genotypes of folate metabolism genes MTHFR and MTRR associated with aneuploidy on chromosomes 13, 16, 18, 21, X and Y in spermatozoa were found out. Degree of implementation: the obtained data were used in the preparation of guidelines "Diagnosis of ejaculate disorders and assisted reproductive technologies: method. decree. For students of IV-V courses of med. Universities and urologists, andrologists and gynecologists”. Field of application: medicine, assisted reproductive technologies.