Novhorodova O. Express-indication of P. aeruginosa by surface plasmon resonance

The thesis is based on the development of express-indication of P. aeruginosa by surface plasmon resonance. The main aspects of the researches were devoted to study of the risk factors in livestock and humane medicine related to the persistence of P. aeruginosa. The results of morphological, cultural, fermentative and biological properties of tested P. aeruginosa isolates were presented. Regarding the thesis, the connection of the adhesive properties of P. aeruginosa with antibiotic resistance has been investigated. Diagnostic antiserum and immunoglobulin have been obtained, which were used during the design of a transducer (chip) of an immunosensor based on a surface plasmon resonance for rapid detection of P. aeruginosa. As a result of studies of transducer’s surface under the conditions of surface treatment of the chip polyalylamine hydrochloride, the sensitivity of the response of the was within 10^1–10^3 cells/cm3 . Sensitivity of surface plasmon resonance immunosensor using a monolayer of antibodies formed on an uncovered surface of gold was from 1000 cells/cm3. The calibration of the elements of the system and the "Plasmon-6" device demonstrated that the response (the minimum fixed refractive angle value) is from 1000 RU, the working serum titer can detect a of 10–103 cells/cm3. There was no probable difference, comparing the data obtained during the study of experimental samples of biomaterials by the classical bacteriological method of sowing and by the method of immunosensory analysis. As a results of the researches, surface plasmon resonance immune biosensor test system for the express diagnostics of bacteria P. aeruginosa is developed. Statistical significance of the analysis grows sharply with the increasing of concentrations. Linearity of the immunosensor is between 10^1–10^6 cells/sm3.