Lamazian H. Development and pharmacological study of antidiabetic activity of Citrullus Colocynthis (L.) Shrad. dry extract.

The thesis is devoted to experimental justification of the effectiveness of the application of Citrullus Colocynthis (L.) Shrad. dry extract. The study of the antidiabetic activity of C. Colocynthis fruit dry extract obtained at Pharmacognosy and Botany Department at Bogomolets National Medical University and standardized for the content of elagic acid was carried out. The presence of expressive antihyperglycemic action of the plant extract on type 1 and 2 diabetes models was determined, by the severity of which the investigated extract outperforms such of a reference agent "Arphasetyn". Based on results of research, the concept of antidiabetic effect of the extract is broadened. Under conditions that mimic the diabetes (the action of prooxidant factors and hyperglycemia), the plant extract prevents death and promotes regeneration of the β-cell’s culture of RIN-m5F line. Experimental studies in vitro and in vivo had shown that one of parts of antidiabetic action of the plant extract is its antioxidant properties, due to which investigated extract has a cytoprotective effect on pancreatic β-cells and exhibits antihyperglycemic effect on the type 1 diabetes model. In addition, on the type 2 diabetes model a dose-dependent antihyperlipidemic effect of Colocynthis extract was revealed, which is manifested in its ability to reduce the level of atherogenic lipids and increase the level of antiatherogenic ones. Results of the study substantiate the expediency of further preclinical and clinical studies of the plant extract and the creation on its basis of an effective medicinal drug for the prevention and correction of prediabetic states, initial stages of type 2 diabetes in therapy regimens.