Mahats D. Study of viral production activity and cultivation conditions of the long-term culture cells FLK BLV on the influence of nanoparticles of Silver, Iron, Zinc and Manganese Dioxide

The dissertation is devoted to the influence of the most biologically widespread nanometals on the cytogenetic indexes and viral production activity of the three sublines long-term culture cells FLK BLV (fetal lamb kidney bovine leukemia virus). It has been determined that nanoparticles of Zinc and Manganese Dioxide in all experimental dilutions exerted cytotoxic influence on cell growth and reproduction. The direct dependence of the intensity of the cytotoxic effect on the dilution of nanometals was established. The cytotoxic effect was significantly enhanced as the concentration of metal increased. The clearly expressed stimulation of proliferation of the cells three sublines under the Silver in dilution of 1:100 was observed. The application of nanoparticles Manganese Dioxide and Zinc in all experimental dilutions suppressed mitotic activity of cell culture FLK 71, FLK SBBL and FLK 50/100 compared to control. According to the results of the research, it has been found that the nanoparticles of Silver in all experimental dilutions caused a decrease the amount of pathological mitoses and stimulated cell proliferation. The destabilization of the karyological indexes of experimental cultures was detected during the cultivation of cells with the Manganese Dioxide and Zinc nanoparticles in all experimental dilutions. The chromosomal analysis showed that the karyotypic features under influence of the Silver and Iron in dilutions of 1:20 and 1:100 were characterized by cells heterogeneity and a small variation of chromosomes amount. For the first time the feasibility of using the Silver nanoparticles in dilution of 1:100 (the initial concentration by metal 432.0 μg/cm3) was developed on basis of obtained data. The addition of Silver 1:100 to the nutrient medium increased the antigen producing activity of cell culture FLK BLV. Experimentally it has been established that the addition of Silver nanoparticles in dilution of 1:100 on the total monolayer of two sublines (FLK SBBL and FLK 71) during 8 consecutive passages increased the antigen producing activity compared to control. The nanoparticles of Silver 1:100 induced an increase of the release of the FLK SBBL antigen twice as much as control. At the same time, the cultivation of culture cells FLK 71 with Silver nanoparticles 1:100 resulted in an increase of amount of virtually-derived antigen by 1.6-fold.