Thesis is devoted to the development and production of complex safe disinfect preparation “Barez” with anti-bactericidal properties through synergic action of vegetable essential oils of fir, eucalyptus and thyme enhanced by quaternary ammonium compounds for aerosol application for the prevention of bacterial etiology-driven animal diseases and for the rehabilitation of premises. Preclinical, clinical and analytical studies of “Barez” bactericidal preparation were conducted. In particular, its bactericidal, fungicidal and acaracidal properties were assessed. A research on preparation’s live toxicological effects on laboratory animals was conducted.
According to the results of the research, the developed preparation belongs to the fourth-grade chemicals on the degree of danger. As was shown by in vitro method of toxic level evaluation of the disinfectant the proliferative capacity of its cell structure is restored after treatment indicating its effectiveness and harmlessness.
The technology of manufacturing the preparation was developed, physical and chemical properties of its application were studied. It was therefore experimentally proved that “Barez” is effective for disinfecting livestock buildings and is safe for animals in 0.5 % concentration. An effective concentration was determined and the duration of its prolonged bactericidal effect was found.
The bactericidal properties have been studied. Effective bactericidal concentrations of the preparation as for the testing cultures Stahylococcus spp., Streptococcus spp., Salmonella spp., Pseudomonas aeruginosa, Proteus vulgariss, Escherichia coli та Clostridium perfringens (effective concentration 0,2 % with 60 min exposition), Васіllus (2,5‒3,0 % - 60 min) were found. A research of the preparation in production conditions was conducted as well as its fungicidal effect on Aspergillus, Penicillium, Fusarium (1,0‒2,0 % - 30 min).
A research on acute toxicity levels with the use of laboratory animals was conducted (LD50 – 5000 ml/kg was determined putting the preparation under fourth grade toxicity level).
A 0.5 % concentration of the preparation in a 0,5 cm3 doesn’t cause visible pathoanatomical changes indicating the low level of toxicity and harm of the preparation in case if its infiltration in the animals’ bodies in production conditions.
It was found that “Barez” bactericidal preparation does not have cumulative and sensitizing properties, does not show excitating and skin-resorptive effects, does not affect hemocytopoiesis and does not expose any suppressive actions on growth and development of animals.
The conducted study of kidneys, spleen, liver, myocardium of control and experimental group of mice showed that differences in the structure of the above-mentioned organs of both groups of animals were not detected.
“Barez” can be used for disinfection in case of diseases of animals, whose pathogens are equated with resistance to E. coli and S. aureus, in 0.5-2.0 % concentrations.
Conducting disinfection with 0.5 % disinfectant solution “Barez” in the premises for keeping pigs in their presence does not negatively affect their health, as evidenced by indices of non-specific resistance factors of animals that remained within the normal range throughout the experiment.
The use of “Barez” (with 0.05-3.0% concentration, working solution spending at 50-100 ml/m3, 30 min exposition, 20 оС temperature and 75 % rH value) for aerosol disinfection of livestock premises allows it to achieve a 100 % effectiveness.
The economic aspects of the use of “Barez” are: reduction of processing time, disinfectant spending, increase in sanitary and hygienic conditions of keeping animals.
The technology for maintaining the preparation and its use were developed.
