In the dissertation work, the level of contamination of poultry facilities and hatching eggs is determined based on the results of experimental research. The range of microorganisms (Bacilus spp., Corynebacterium spp., Proteus vulgaris, Staphylococcus spp., Саndida spp., and Escherichia coli), which are most often isolated from poultry facilities of the incubator, has been studied. In the comparative aspect, the disinfecting effect of modern disinfectants “Iodis”, “Polydez”, “Virosan”, and “Sterylii AB” has been evaluated and the optimal modes of their use for pre-incubation and incubation treatment of chicken eggs have been determined. The hatchability of eggs with the use of disinfectant “Iodis” in a concentration of 0.5% does not exceed 69.2%, and in a concentration of 1.0% — 70.2%. When disinfecting hatching eggs with a combination of “Iodis” (1.0%) and “Polydez” (0.1%), hatchability increases to 75.6%. It has been proven that disinfectants “Polydez” in 0.1% concentration and “Virosan” in 0.1% concentration have a bactericidal effect on the bacterial microflora of hatching egg shells and do not have a negative effect on their incubation qualities. It has been determined that the use of ozone and UV radiation is effective for the purpose of decontamination of poultry facilities. When the surface of hatching eggs was treated with ozone at a concentration of 0.1–0.5 and 1 g/m3 for 60 min, no negative effect on the embryonic development of chickens was detected. It was found that 22-day-old turkey eggs with well-developed embryos during the incubation period have an average shell surface temperature of 31.7 °С, with retarded embryos — 30.1 °С, with dead embryos — 29.4 °С. 24-day-old turkey eggs with well-developed embryos during the incubation period have a shell surface temperature of 33.8 °С, with retarded embryos — 33.1 °С, with dead embryos — 29.3 °С. It has been proven that the thermometric method of determining live embryos during incubation makes it possible to remove eggs with dead embryos before transferring eggs for hatching, which allows to obtain viable young and to improve the sanitary condition of hatching halls and the environment around the incubator. The main shortcomings in the operation of the ventilation system of “Universal” incubators, namely the excessive difference of microclimatic conditions on the diagonal of each cabinet, have been identified, and reasonable recommendations for their elimination are given. It has been found that the level of microbial contamination of cardboard cells, even after a single use for transporting hatching eggs to incubation sites, is very high, and their reuse is a source of microbial and fungal microflora, and the degree of contamination increases almost 40 times.
