Ravshanov T. Pharmacological study of prostateprotective action of the combined composition suppositories

Dissertation dedicated to the complex pharmacological study prostatoprotective action of combined composition suppositories with indole-3-carbinol and meloxicam. In rats with prostatic hyperplasia due to the development of hyperprolactinemia induced by the introduction of sulpiride, an increase in iron mass (approximately 30%) was detected. Light-optical studies of the sections of the gland showed clear signs of focal hyperplasia of the prostatic and duct epithelium with inflammatory processes in the stroma. There was a twofold decrease in androgenization of the organism with a decrease in the concentration of fructose (by 43.7 %), impaired spermatogenesis (a decrease in the concentration of spermatozoa by 31.2%, among which almost doubled the proportion of abnormal forms), changes in acid-base balance of suspension, gonadotoxic effects on the morphostructure of the testes. The enzyme-linked immunosorbent assay established hormonal balance disorders. Thus, the concentration of total testosterone and its free form decreased statistically significantly (by 44.8 and 11.7%, respectively). Correction with the help of the proposed CCS reduced the relative mass of the gland and morphological features of the pathology, in particular, hypertrophy of the acini of prostatic glands, minimization of the manifestations of hyperplasia of the glandular epithelium, normalization of phosphatase activity was observed. The effect of correction therapy with CCS was to reduce the concentration of all hormones: total T, the absolute amount and proportion of free T and E2 in comparison with the data of model pathology. According to these results, the CCS suppositories were dominated by the effect of the reference drug with pumpkin seed oil. The decrease in the activity of 5α-reductase (34.4 %) and the content of androgen receptors (76.0 %), in our opinion, is the result of the specific corrective activity of indole-3-carbinol. By the effect of a positive effect on the morphological manifestations of testosterone propionate induced prostate hyperplasia, metabolic indicators and integral indicators of cellular changes of the prostate gland, suppositories of the combined composition are dominated by the comparator –suppositories with pumpkin seed oil. The use of these models of pathology made it possible to evaluate qualitatively the mechanism of action of suppositories of the combined composition. The results of the study confirm the presence of hormone-modulating activity and the positive effect of the proposed the CCS suppositories on the important pathogenetic links of prostatic hyperplasia. The successful combination of pharmacological and technological properties of CCS suppositories with I3C meets current requirements for the medicinal products of this therapeutic group.