Dovha N. Morphological changes of the retina under the influence of paclitaxel and under the conditions of their correction

The aim is to study the ultrastructural changes in the retina in the correction of Paclitaxel-induced retinotoxicity using Armadin in the experiment. In the run of experiment 72 white rats received intraperitoneal injections of Paclitaxel (Actavis, Romania) dosed 2 mg/kg of body weight, 4 times every 24 hours; the total dosage equaled 8 mg/kg. the following steps was injecting 48 animals with Armadin at the dosage of 10 mg/kg of body weight (the control group of 24 animals received intraperitoneal injections of water for injection). Pieces of retina were taken to be studied using the electronic microscope. The scientific novelty of the obtained results is the first introduced information on morphometric analysis of retinal layers in intact animals, after paclitaxel injection, and after correction of 2-ethyl-6-methyl-3-hydroxypyridine succinate in different terms, but is a detailed morphological characteristic of the retina with the development of its morpho-functional changes with prolonged exposure to paclitaxel. It has been found out, that the positive effect of Armadin in Paclitaxel-induced retinopathy manifests itself since the 7th-15th day through the activation of pigment cells, the distinct contours of membranous discs in rods and cones; the increased volume of neuron perikaryons; mitochondria have well-distinctive membranes and cristae; the synaptic contacts between the neurons and their processes in the outer plexiform layer (spherules) are well outlined. In the retina capillaries in the cytoplasm of endothelial cells, along the luminal plasma membrane micropinocytotic vesicles are concentrated. On the 21st-27th day the state of the basal complex, of bipolar and ganglion neurons, of inner plexiform and neuron fiber layers improve. Starting from the 60th day there appear the first signs of Armadin lack. In the photoreceptor layer, the outer segments of rods and cones have areas of narrowing and expansion. The capillaries of the outer capillary plexus have an enlarged lumen. In the inner reticular layer, the cross sections of the processes of neurons of adjacent layers are deformed. Axons of neurons are expanded and deformed. On the 90th-120th day the destabilization of cell membranes keeps developing, the mitochondria manifest the damages of inner membrane; the radial glial cells become more active.