The dissertation is devoted to solving an urgent scientific and practical problem of modern dentistry – clinical and laboratory substantiation of the choice of the optimal method of periodontal treatment using a drug with chronotype features.
The purpose of the work was to increase the effectiveness of treatment of patients with manifestations of the periodontitis stage II grade B, depending on the chtonotype.
The average values of the OHI-S hygiene index among participants of groups II, III and IV reflected an unsatisfactory level of oral hygiene compared to the control group with a good level of hygiene. Evaluation of the PMA index revealed an average degree of gingivitis severity in groups II, III and IV and mild degree of gingivitis among participants of the control group. The mean values of the periodontal Russell index among participants of groups II, III and IV corresponded to the severe degree of periodontal pathology. In the control group it corresponded to the initial degree of periodontal pathology. The bleeding gingival index of BOP among participants of group II was 0,266±0,009, among participants of group III – 0,273±0,007, among group IV – 0,278±0,006. Participants in the control group demonstrated an index of 0,031±0,003.
Analyzing the results obtained, it was found that the average depth of periodontal pockets in participants of group I was 0,167±0,012 mm, in participants of group II – 2,556±0,124 mm, among participants of group III – 2,506±0,091 mm, and among participants of group IV – 2,354±0,09 mm. The mean loss of epithelial attachment in participants of group I was 0,187±0,015 mm, in participants of group II – 2,632±0,131 mm, among participants of group III – 2,616±0,103 mm, and among participants of group IV – 2,418±0,117 mm.
In our study, statistically significant decreased values of the physical and mental health scale.
The results showed that Per1 demonstrates the same rhythmic expression, which peaks in the early morning (at 07:00) and decreases in the evening, regardless of chronotype, age, and gender. The evening predominance of Clock expression was found regardless of sex, and age. Evening expression of Clock was higher in individuals with an evening chronotype than with a morning chronotype.
The expression of Bmal1 showed a maximum level in the evening (at 19:00) regardless of chronotype, age, and gender .The level of Cry1 expression was higher at 19:00 regardless of chronotype, age, and gender. Chronotype, gender and age did not have any effect on Per1, Cry1, Bmal1 expression.
After analyzing the data in participants of groups II, II and IV obtained, we found that the expression levels of the circadian clock genes Per1, Clock, Bmal1, Cry1 in different groups do not coincide with each other, sometimes have a chaotic order and do not follow the patterns of normal circadian gene expression that have been studied in many studies. We can assume that this variation in the values is caused by the effect of desynchronization on the mechanisms of circadian regulation in buccal epithelial cells.
