Novokhatska T. Application of RNA-interference therapy targeted to protein kinase C for correction of vascular dysfunction under arterial hypertension

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0413U005584

Thesis Registration Form

0413U005584.pdf

Applicant for

Specialization

  • 14.03.05 - Фармакологія

02-10-2013

Specialized Academic Board

Д 26.550.01

Institute of Pharmacology and Toxicology of the National Academy of Medical Sciences of Ukraine

Essay

The dissertation is devoted to the efficacy of PKC-delta gene silencing in the experimental therapy of vascular abnormalities related to ionic channelopathy, vascular hypercontactility and high blood pressure in spontaneously hypertensive rats (SHRs) using RNA-interference (RNAi) technique: small interfering RNA (siRNAs) and plasmid-based siRNAs (plRNAs). The expression profile of PKC-delta mRNA transcripts and protein level in SHRs aorta were significantly decreased after PKC-delta RNAi using both siRNAs and plRNAs. The repairing effects of RNAi therapy on BKCa channels activity and vascular relaxant responses were studied. It may be likely that PKC-delta gene suppression by RNA-interference appears to be a good approach to inactivate unwanted gene encoding function and to normalize vascular tone damaged in arterial hypertension.

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